Paragon Laboratories
12649 Richfield Ct.
Livonia, MI 48150
734.462.3900 Phone

TTO Semivolatile Organic Compounds in Wastewater Analysis by EPA 625.1

Summary

EPA 625.1 is used to determine the concentrations of Semivolatile Organic Compounds (SVOCs) in wastewater. SVOCs can be classified into two subcategories of compounds known as Base/Neutral and Acid (BNA) extractable compounds. Phenolic compounds make up the Acid extractables; Polynuclear Aromatic (PNA) compounds are a subset of the Base/Neutral compounds.

Technique

EPA 625.1 is performed using separatory funnel liquid-liquid extraction and gas chromatography/mass spectrometry detection (GC/MS).

Holding Time

Samples must be extracted within 7-days of collection. Samples not extracted within this period must be discarded and replaced. Sample extracts must be analyzed within 40 days of extraction.

Container and Preservative

Collect all samples in (2) 1-L amber glass bottles fitted with a fluoropolymer-lined screw cap. Bottles must NOT be rinsed with the sample before collection. Samples must be kept refrigerated at ≤6°C and protected from light during compositing. Ice or refrigerate samples at >0°C to ≤6°C from the time of collection, but do NOT freeze. If residual chlorine is present, add a dechlorinating agent (e.g., 80 mg/L sodium thiosulfate, ascorbic acid, or sodium sulfite) and mix well.

Shipping/Transport Instructions

Samples must be protected from light and chilled to >0°C to ≤6°C at collection and kept at >0°C to ≤6°C until extracted. Field samples must be packaged for shipment with sufficient ice to ensure that they will be >0°C to ≤6 °C upon arrival at the laboratory.

Analytes and Reporting Limits

Analytes routinely reported are listed below.

 

Analyte CAS Number Reporting Limit
2,3,7,8-TCDD (as TIC) 1746-01-6 µg/L
N-Nitrosodimethylamine 62-75-9 10 µg/L
Phenol 108-95-2 10 µg/L
Bis(2-chloroethyl)ether 111-44-4 10 µg/L
2-Chlorophenol 95-57-8 10 µg/L
Bis(2-chloroisopropyl) ether 39638-32-9 10 µg/L
N-Nitrosodi-n-propylamine 621-64-7 10 µg/L
Hexachloroethane 67-72-1 10 µg/L
Nitrobenzene 98-95-3 10 µg/L
Isophorone 78-59-1 10 µg/L
2-Nitrophenol 88-75-5 10 µg/L
2,4-Dimethylphenol 105-67-9 10 µg/L
Bis(2-chloroethoxy)methane 111-91-1 10 µg/L
2,4-Dichlorophenol 120-83-2 10 µg/L
1,2,4-Trichlorobenzene 120-82-1 10 µg/L
Naphthalene 91-20-3 10 µg/L
Hexachlorobutadiene 87-68-3 10 µg/L
4-Chloro-3-methylphenol 59-50-7 10 µg/L
Hexachlorocyclopentadiene 77-47-4 10 µg/L
2,4,6-Trichlorophenol 88-06-2 10 µg/L
2-Chloronaphthalene 91-58-7 10 µg/L
Dimethyl phthalate 131-11-3 10 µg/L
Acenaphthylene 208-96-8 10 µg/L
2,6-Dinitrotoluene 606-20-2 10 µg/L
Acenaphthene 83-32-9 10 µg/L
2,4-Dinitrophenol 51-28-5 10 µg/L
4-Nitrophenol 100-02-7 10 µg/L
2,4-Dinitrotoluene 121-14-2 10 µg/L
Diethyl phthalate 84-66-2 10 µg/L
Fluorene 86-73-7 10 µg/L
4-Chlorophenyl phenyl ether 7005-72-3 10 µg/L
N-Nitrosodiphenylamine 86-30-6 10 µg/L
2-Methyl-4,6-dinitrophenol 534-52-1 10 µg/L
Azobenzene (for 1,2-DPH) 103-33-3 10 µg/L
4-Bromophenyl phenyl ether 101-55-3 10 µg/L
Hexachlorobenzene (HCB) 118-74-1 10 µg/L
Pentachlorophenol 87-86-5 10 µg/L
Phenanthrene 85-01-8 10 µg/L
Anthracene 120-12-7 10 µg/L
Di-n-butyl phthalate 84-74-2 10 µg/L
Fluoranthene 206-44-0 10 µg/L
Benzidine 92-87-5 10 µg/L
Pyrene 129-00-0 10 µg/L
Butyl benzyl phthalate 85-68-7 10 µg/L
Benzo(a)anthracene 56-55-3 10 µg/L
Chrysene 218-01-9 10 µg/L
3,3'-Dichlorobenzidine 91-94-1 10 µg/L
Bis(2-ethylhexyl)phthalate 117-81-7 10 µg/L
Di-n-octyl phthalate 117-84-0 10 µg/L
Benzo(b)fluoranthene 205-99-2 10 µg/L
Benzo(k)fluoranthene 207-08-9 10 µg/L
Benzo(a)pyrene 50-32-8 10 µg/L
Dibenzo(ah)anthracene 53-70-3 10 µg/L
Indeno(1,2,3-cd)pyrene 193-39-5 10 µg/L
Benzo(ghi)perylene 191-24-2 10 µg/L