Paragon Laboratories
12649 Richfield Ct.
Livonia, MI 48150
734.462.3900 Phone

TTO Pesticides and Polychlorinated Biphenyls in Wastewater Analysis by EPA 608.3


EPA 608.3 is used to determine the concentrations of Pesticides and Polychlorinated Biphenyls (PCBs) in wastewater.


EPA 608.3 is performed using separatory funnel liquid-liquid extraction and gas chromatography/electron capture detection (GC/ECD).

Holding Time

Samples must be extracted within 7-days of collection. Samples not extracted within this period must be discarded and replaced. If the sample will not be extracted within 72 hours of collection, check the pH and adjust to a range of 5.0 to 9.0; record the volume of sodium hydroxide (NaOH) or sulfuric acid (H2SO4) used. Sample extracts must be analyzed within 40 days of extraction.

Container and Preservative

Collect all samples in (2) 1-L amber glass bottles fitted with a fluoropolymer-lined screw cap. Bottles must NOT be rinsed with the sample before collection. Samples must be kept refrigerated at ≤6°C and protected from light during compositing. Ice or refrigerate samples at >0°C to ≤6°C from the time of collection, but do NOT freeze. If residual chlorine is present and aldrin is to be determined, add a dechlorinating agent (e.g., 80 mg/L sodium thiosulfate, ascorbic acid, or sodium sulfite) and mix well.

Shipping/Transport Instructions

Samples must be protected from light and chilled to >0°C to ≤6°C at collection and kept at >0°C to ≤6 °C until extracted. Field samples must be packaged for shipment with sufficient ice to ensure that they will be >0°C to ≤6 °C upon arrival at the laboratory.

Analytes and Reporting Limits

Analytes routinely reported are listed below.


Analyte CAS Number Reporting Limit
Aldrin 309-00-2 0.1 µg/L
alpha-BHC 319-84-6 0.1 µg/L
beta-BHC 319-85-7 0.1 µg/L
delta-BHC 319-86-8 0.1 µg/L
Lindane (gamma-BHC) 58-89-9 0.1 µg/L
Chlordane (as alpha- + gamma-) 57-74-9 0.01 µg/L
Chlordane, Technical (if required) 57-74-9 0.1 µg/L
4,4'-DDD 72-54-8 0.1 µg/L
4,4'-DDE 72-55-9 0.1 µg/L
4,4'-DDT 50-29-3 0.1 µg/L
Dieldrin 60-57-1 0.1 µg/L
Endosulfan I 959-98-8 0.1 µg/L
Endosulfan II 33213-65-9 0.1 µg/L
Endosulfan sulfate 1031-07-8 0.1 µg/L
Endrin 72-20-8 0.1 µg/L
Endrin aldehyde 7421-93-4 0.1 µg/L
Heptachlor 76-44-8 0.1 µg/L
Heptachlor epoxide 1024-57-3 0.1 µg/L
Toxaphene 8001-35-2 1 µg/L
PCB Aroclor 1016 12674-11-2 0.1 µg/L
PCB Aroclor 1221 11104-28-2 0.1 µg/L
PCB Aroclor 1232 11141-16-5 0.1 µg/L
PCB Aroclor 1242 53469-21-9 0.1 µg/L
PCB Aroclor 1248 12672-29-6 0.1 µg/L
PCB Aroclor 1254 11097-69-1 0.1 µg/L
PCB Aroclor 1260 11096-82-5 0.1 µg/L
Total PCBs 1336-36-3 0.1 µg/L